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KMID : 0043320170400030356
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Archives of Pharmacal Research
2017 Volume.40 No. 3 p.356 ~ p.363
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Chitosan?ceramide coating on gold nanorod to improve its physiological stability and reduce the lipid surface-related toxicity
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Battogtokh Gantumur
Gotov Oyuntuya
Ko Young-Tag
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Abstract
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Gold nanoparticles are promising materials for many applications that include imaging, drug delivery, and photothermal therapy. However, AuNPs can be unstable and/or toxic. We purposed to improve the stability and reduce toxicity of gold nanorods (AuNR) upon coating with biocompatible polymer, chitosan?ceramide (CS?CE), without replacing the original layer, CTAB. CS?CE-coated AuNR was prepared by simple mixing for 24 h and purified by centrifugation. The coating was confirmed by UV?Vis absorption analysis and surface charge and size measurement. We prepared nanorods with CS or CS?CE coating at two different concentrations (5 and 10% AuNR), the resulting in larger nanorods with a more positive surface-charge than that of AuNR. We investigated the UV-absorption and protein adsorption of the polymer coated nanorods. Based on the protein adsorption, AuNR-CS?CE was found to be more stable under physiological conditions than AuNR-CS. The cell internalization assay revealed that Hela cells internalized higher amounts of AuNR-CS?CE than that of AuNR-CS. Cytotoxicity study revealed that AuNR-CS?CE has lower toxicity than AuNR against HeLa cells. The CS?CE coating improved the stability of AuNR under physiological conditions via the hydrophobic interactions between the AuNR lipid surface and the ceramide anchor in the CS backbone as well as.
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KEYWORD
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Polymer coating, Gold nanorod, Chitosan, Ceramide, Colloidal stability
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